A Chemoenzymatic Histology Method for O‐GlcNAc Detection. (5th December 2017)

• Record Type: Journal Article
• Authors: Aguilar, Aime Lopez; Hou, Xiaomeng; Wen, Liuqing; Wang, Peng G.; Wu, Peng
• Is Part Of: Chembiochem. Volume 18:Number 24(2017)
• Page Start: 2416
• Page End: 2421
• Publication Date: 2017-12-05
• Subjects: chemoenzymatic detection -- GalT1 -- glycan labeling -- histology -- O-GlcNAc; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201700515
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Modification of nuclear and cytoplasmic proteins by the addition or removal of O‐GlcNAc dynamically impacts multiple biological processes. Here, we present the development of a chemoenzymatic histology method for the detection of O‐GlcNAc in tissue specimens. We applied this method to screen murine organs, uncovering specific O‐GlcNAc distribution patterns in different tissue structures. We then utilized our histology method for O‐GlcNAc detection in human brain specimens from healthy donors and donors with Alzheimer's disease and found higher levels of O‐GlcNAc in specimens from healthy donors. We also performed an analysis using a multiple cancer tissue array, uncovering different O‐GlcNAc levels between healthy and cancerous tissues, as well as different O‐GlcNAc cellular distributions within certain tissue specimens. This chemoenzymatic histology method therefore holds great potential for revealing the biology of O‐GlcNAc in physiopathological processes. Abstract : O‐GlcNAcylation dynamically impacts multiple biological processes : We developed a chemoenzymatic histology method for O‐GlcNAc detection and applied it to examine O‐GlcNAcylation in tissue specimens from healthy donors and patients. Specific O‐GlcNAc distribution patterns were uncovered, underlining the utility of this method for studying O‐GlcNAc biology in physiopathological processes.