Low sensitivity and frequent cross‐reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis. Issue 1 (7th December 2017)

• Record Type: Journal Article
• Authors: Garcia, Hector H.; Castillo, Yesenia; Gonzales, Isidro; Bustos, Javier A.; Saavedra, Herbert; Jacob, Louis; Del Brutto, Oscar H.; Wilkins, Patricia P.; Gonzalez, Armando E.; Gilman, Robert H.
• Other Names: Tsang Victor C.W. investigator.; Rodriguez Silvia investigator.; Martinez Manuel investigator.; Alvarado Manuel investigator.; Porras Miguel investigator.; Vargas Victor investigator.; Ccjuno Alfredo investigator.; Verastegui Manuela investigator.; Zimic Mirko investigator.; Mayta Holger investigator.; Guerra Cristina investigator.; Castro Yagahira investigator.; Lopez Maria T. investigator.; Gavidia Cesar M. investigator.; Gomez Luis investigator.; Moyano Luz M. investigator.; Gamboa Ricardo investigator.; Muro Claudio investigator.; Vilchez Percy investigator.; Nash Theodore E. investigator.; Mahanty Siddhartha investigator.; Noh John investigator.; Handali Sukwan investigator.; Friedland Jon investigator.
• Is Part Of: Tropical medicine & international health. Volume 23:Issue 1(2018)
• Page Start: 101
• Page End: 105
• Publication Date: 2017-12-07
• Subjects: Taenia solium -- cysticercosis -- neurocysticercosis -- antibody -- ELISA -- Peru; Tænia solium -- cysticercose -- neurocysticercose -- anticorps -- ELISA -- Pérou; Tropical medicine -- Periodicals; Public health -- Periodicals; 616.988
• DOI: 10.1111/tmi.13010
• Languages: English
• ISSNs: 1360-2276

Abstract

Abstract: Objective: To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa ® and Ridascreen ®, for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP‐EITB (electro‐immunotransfer blot assay using lentil‐lectin purified glycoprotein antigens). Methods: Archive serum samples from patients with viable NCC ( n = 45) or resolved, calcified NCC ( n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa ® and Ridascreen ® . All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP‐EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. Results: Compared to LLGP‐EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross‐reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross‐reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). Conclusions: The performance of Novalisa ® and Ridascreen ® was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.