Determining the optimal protocol for preparing an acellular scaffold of tissue engineered small‐diameter blood vessels. Issue 2 (8th March 2017)

• Record Type: Journal Article
• Authors: Pu, Lei; Wu, Jian; Pan, Xingna; Hou, Zongliu; Zhang, Jing; Chen, Wenmin; Na, Zhuhui; Meng, Mingyao; Ni, Haiyan; Wang, Liqiong; Li, Yaxiong; Jiang, Lihong
• Is Part Of: Journal of biomedical materials research. Volume 106:Issue 2(2018)
• Page Start: 619
• Page End: 631
• Publication Date: 2017-03-08
• Subjects: tissue engineering -- decellularization -- extracellular matrix -- small‐diameter blood vessels; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.b.33827
• Languages: English
• ISSNs: 1552-4973

Abstract

Abstract: Although detergent‐based decellularization protocols have been widely used to obtain a natural extracellular matrix (ECM) scaffold in tissue engineering, some key challenges still exist. To achieve an optimum natural decellularized scaffold for the construction of tissue‐engineered small‐diameter blood vessels (TEBV), porcine carotid arteries (PCAs) were decellularized by combining sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC) and Triton X‐100 (Triton) in different concentrations. Tissue samples were processed and their histological, biochemical and biomechanical characteristics were investigated. Results showed that only two methods 0.5% (SDS + SDC) and 1% (SDS + SDC) could completely remove of the cellular contents and preserve the native ECM architecture. Furthermore, 1% (SDS + SDC) based methods acquire preferable porosity and suitable mechanical strength. Residual Triton in the ECM scaffold holds intensive cytotoxity. In conclusion, 1%(SDS + SDC) based method can obtain a superior PCAs scaffold for the construction of TEBV. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 619–631, 2018.