NFκB transcription factor (p65) immunohistochemistry in leprosy dermal microvasculature. (December 2017)

• Record Type: Journal Article
• Authors: Silva, Luciana Mota; Hirai, Kelly Emi; de Sousa, Jorge Rodrigues; de Souza, Juarez; Dias, Leônidas Braga; Carneiro, Francisca Regina Oliveira; Aarão, Tinara Leila de Souza; Fuzii, Hellen Thais; Quaresma, Juarez Antonio Simões
• Is Part Of: Microbial pathogenesis. Volume 113(2017)
• Page Start: 427
• Page End: 431
• Publication Date: 2017-12
• Subjects: Leprosy -- Endothelial adhesion molecules -- NFκB; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.11.027
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Leprosy caused by Mycobacterium leprae is characterized by a spectrum of clinical manifestations that are determined by the predominant immunological profile of the host. The recruitment of leukocytes to the sites of injury can influence the development of these profiles. Cell adhesion molecules such as ICAM-1, VCAM-1 and CD62E participate in this process and their expression is regulated by transcriptions factors such as NFκB. To correlate the expression of cell adhesion molecules and NFκB (p65) in leprosy lesions, 30 skin biopsies of patients with leprosy [16 with the tuberculoid (TT) or borderline tuberculoid (BT) forms and 14 with the lepromatous (LL) or borderline lepromatous (BL) forms] were analyzed by immunohistochemistry. A larger mean number of cells expressing VCAM-1 (BT/TT: 18.28 ± 1.4; BL/LL: 10.67 ± 1.2; p = 0.0002), ICAM-1 (BT/TT: 9.92 ± 1.1; BL/LL: 5.87 ± 1.0; p = 0.0084) and CD62E (BT/TT: 13.0 ± 1.5; BL/LL: 2.58 ± 0.3; p = 0.0001) were observed in BT and TT lesions. The mean number of cells expressing NFκB was similar in the two clinical forms (BT/TT: 2.21 ± 2.7; BL/LL: 2.35 ± 3.1; p = 0.9285). No significant correlation was observed between expression of the transcription factor and adhesion molecules analyzed. The synthesis of ICAM-1, VCAM-1 and CD62E depends on the activation of NFκB, which acts synergistically with other transcription factors. Adequate activation of intracellular signaling pathways results in the production of endothelial adhesion molecules, contributing to the recruitment of cells to the site of injury and thus eliciting an effective inflammatory response in the elimination of the bacillus. Highlights: Leprosy is characterized by clinical manifestations that are determined by the immunological profile of the host. Recruitment of blood leukocytes to the lesion site is important for the pattern of immune response. Adequate activation of intracellular signaling pathways results in the production of endothelial adhesion molecules. These molecules are crucial for the recruitment of leukocytes to the focus of infection.