In silico design, cloning, expression and immunologic evaluation of ED fusion protein of NT H. influenzae. (December 2017)

• Record Type: Journal Article
• Authors: Behrouzi, Ava; Bouzari, Saeid; Oloomi, Mana; Fateh, Abolfazl; Vaziri, Farzam; Afrough, Parviz; Amanzadeh, Amir; Siadat, Seyed Davar
• Is Part Of: Microbial pathogenesis. Volume 113(2017)
• Page Start: 472
• Page End: 479
• Publication Date: 2017-12
• Subjects: Nontypeable Haemophilus influenza -- Fusion protein ED -- Vaccine; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.11.007
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Infections due to nontypeable Haemophilus influenzae (NTHi) are important causes of child mortality throughout the world. Given the lack of effective vaccines for these strains and the spread and prevalence of these infections in the world, it is necessary to design novel vaccine candidates against these strains. D and E proteins are conserved membrane-specific lipoproteins among encapsulated and non-encapsulated H. influenza strains, which, according to the exposure surface and conservation degree between both strains, can be considered as vaccine candidates suitable for studies. This research was conducted to design a recombinant truncated fusion protein ED. Vaccination of BALB/c mice with recombinant truncated fusion protein ED showed high level of protective responses against NTHi. There were also strong responses of IgG and its subclasses (especially IgG1) as well as high titer levels of IL-4. A mixture of responses was observed considering IgG2a and INF-γ antibody titers, but the dominant response was toward Th2. According to the obtained results and the importance of humoral immunity in the immune system and vaccines production, it could be concluded that the produced recombinant construct can be used as a suitable vaccine candidate against NTHi or together with other carrier proteins. Highlights: The present study aimed to design a novel fusion factor as a vaccine candidate against non-encapsulated Haemophilus influenzae. The results of this study showed a significant increase in the levels of IgG antibodies and its subclasses of IgG1 and IgG2a, as well as the level of IL-4 as a representative of the humoral immune response (Th2). Due to the high protective level of increased titers, the new designed fusion protein seems to have potential for the novel vaccine candidate against non-encapsulated Haemophilus influenzae .