Bioengineering Novel Floating Nanoparticles for Protein and Drug Delivery. (2016)

• Record Type: Journal Article
• Authors: DasSarma, Priya; Karan, Ram; Kim, Jong-Myoung; Pecher, Wolf; DasSarma, Shiladitya
• Is Part Of: Materials today. Volume 3:Number 2(2016)
• Page Start: 206
• Page End: 210
• Publication Date: 2016
• Subjects: Gas vesicle naoparticles (GVNPs) -- vaccine -- luciferase -- Gaussia princeps -- enzyme display; Materials science -- Congresses -- Periodicals; 620.1
• DOI: 10.1016/j.matpr.2016.01.058
• Languages: English
• ISSNs: 2214-7853

Abstract

Abstract: Gas vesicle nanoparticles (GVNPs) are hollow protein nanoparticles produced by Halobacterium sp. NRC-1 which are beingengineered for protein delivery. To advance thebioengineering potential of GVNPs, a strain of NRC-1 deleted for the gvp C gene (Δ gvp C) was constructed and a synthetic gene coding for Gaussia princeps luciferase wasfused to an abbreviated gvp C gene on an expression plasmid. When introduced into theΔgvpC strain, an active GvpC-luciferase fusion protein bound to GVNPs resulted. These results represent both a technical improvement in the GVNP display system and its expansionforthe display of active enzymes.