Droplet-based digital PCR and next generation sequencing for monitoring circulating tumor DNA: a cancer diagnostic perspective. (2nd January 2018)

• Record Type: Journal Article
• Authors: Postel, Mathilde; Roosen, Alice; Laurent-Puig, Pierre; Taly, Valerie; Wang-Renault, Shu-Fang
• Is Part Of: Expert review of molecular diagnostics. Volume 18:Number 1(2018)
• Page Start: 7
• Page End: 17
• Publication Date: 2018-01-02
• Subjects: Droplet-based digital PCR -- NGS -- circulating tumor DNA -- early cancer diagnosis -- mutation -- DNA methylation -- biomarker; Molecular diagnosis -- Periodicals; 616.0758205
• DOI: 10.1080/14737159.2018.1400384
• Languages: English
• ISSNs: 1473-7159

Abstract

ABSTRACT: Introduction : Early detection of cancers through the analysis of ctDNA could have a significant impact on morbidity and mortality of cancer patients. However, using ctDNA for early cancer diagnosis is challenging partly due to the low amount of tumor DNA released in the circulation and its dilution within DNA originating from non-tumor cells. Development of new technologies such as droplet-based digital PCR (ddPCR) or optimized next generation sequencing (NGS) has greatly improved the sensitivity, specificity and precision for the detection of rare sequences. Areas covered : This paper will focus on the potential application of ddPCR and optimized NGS to detect ctDNA for detection of cancer recurrence and minimal residual disease as well as early diagnosis of cancer patients. Expert commentary : Compared to tumor tissue biopsies, blood-based ctDNA analyses are minimally invasive and accessible for regular follow-up of cancer patients. They are also described as a better picture of patients' pathology allowing to highlight both tumor heterogeneity and multiple tumor sites. After a brief introduction on the application of the follow-up of ctDNA using genetic or epigenetic biomarkers for prognosis and surveillance of cancer patients, potential perspectives of using ctDNA for early diagnosis of cancers will be presented.