Integrated process of extraction and purification of betanin from Opuntia ficus-indica using aqueous two-phase systems based on THF and sodium salts. Issue 5 (24th March 2018)

• Record Type: Journal Article
• Authors: Santos, Ricardo P.; Souza, Luana M.; Balieiro, Acenini L.; Soares, Cleide M. F.; Lima, Álvaro S.; Souza, Ranyere L.
• Is Part Of: Separation science and technology. Volume 53:Issue 5(2018)
• Page Start: 734
• Page End: 744
• Publication Date: 2018-03-24
• Subjects: Opuntia ficus-indica -- betanin -- extraction -- purification -- aqueous two-phase systems; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2017.1397022
• Languages: English
• ISSNs: 0149-6395

Abstract

ABSTRACT: This research present an integrated process for the extraction and purification of betanin from fruit of Opuntia ficus-indica . The extraction process was evaluated using different solvents (water, acetonitrile, ethanol and tetrahydrofuran), and then integrated into the purification process using aqueous two-phase systems (ATPS). The optimum conditions for extraction was with THF and the purification conditions determined to be 43 wt% of THF and 6 wt% of sodium citrate buffer (pH 5.5) at 25°C. A purification factor of 13.7 ± 0.5 fold, with a partition coefficient of betanin for salt-rich phase and the antioxidant capacity was increased ≈ 65%.