Incorporation of a Ligand Peptide for Immune Inhibitory Receptor LAIR‐1 on Biomaterial Surfaces Inhibits Macrophage Inflammatory Responses. Issue 24 (30th October 2017)

• Record Type: Journal Article
• Authors: Kim, Yoon Kyung; Chu, Shu‐Hui; Hsieh, Jessica Y.; Kamoku, Cody M.; Tenner, Andrea J.; Liu, Wendy F.; Wang, Szu‐Wen
• Is Part Of: Advanced healthcare materials. Volume 6:Issue 24(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-10-30
• Subjects: collagen peptides -- immunomodulatory biomaterials -- inflammation -- LAIR‐1 -- macrophages; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201700707
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: Leukocyte‐associated immunoglobulin‐like receptor‐1 (LAIR‐1) is an inhibitory receptor broadly expressed on immune cells, with its ligands residing within the extracellular matrix protein collagen. In this study, surfaces are modified with a LAIR‐1 ligand peptide (LP), and it is observed that macrophages cultured on LAIR‐1 LP‐conjugated surfaces exhibit significantly reduced secretion of inflammatory cytokines in response to proinflammatory stimuli that reflect an injured environment. These downregulated mediators include TNF‐α, MIP‐1α, MIP‐1β, MIP‐2, RANTES, and MIG. Knockdown of LAIR‐1 using siRNA abrogates this inhibition of cytokine secretion, supporting the specificity of the inhibitory effect to this receptor. These results are the first to demonstrate that integration of LAIR‐1 ligands with biomaterials could suppress inflammatory responses. Abstract : Modification of material surfaces with a ligand peptide for immune inhibitory receptor, leukocyte‐associated immunoglobulin‐like receptor‐1 (LAIR‐1), significantly reduces the secretion of inflammatory cytokines of macrophages in response to proinflammatory stimuli. The LAIR‐1 ligand peptide is derived from the extracellular matrix protein collagen, and the incorporation of this ligand peptide to biomaterial surfaces may be a strategy to suppress local inflammatory responses toward implanted materials.