Rapid and simplified synthesis of [18F]Fluoromisonidazole and its use in PET imaging in an experimental model of subarachnoid hemorrhage. (February 2018)

• Record Type: Journal Article
• Authors: Fernandez-Maza, Laura; Egea-Guerrero, Juan Jose; Balcerzyk, Marcin; Civantos-Jubera, Gemma; Gordillo-Escobar, Elena; Vilches-Arenas, Angel; Fernandez-Gomez, Isabel; Parrado-Gallego, Angel; Murillo-Cabezas, Francisco
• Is Part Of: Applied radiation and isotopes. Volume 132(2018:Feb.)
• Page Start: 79
• Page End: 84
• Publication Date: 2018-02
• Subjects: [18F]FMISO -- Hypoxia -- Subarachnoid hemorrhage -- PET/CT -- Small animal model of SAH; Radiology -- Periodicals; Radiation -- Industrial applications -- Periodicals; Nuclear chemistry -- Periodicals; Internet resource; Periodical; 660.298
• DOI: 10.1016/j.apradiso.2017.09.035
• Languages: English
• ISSNs: 0969-8043

Abstract

Abstract: Cerebral damage secondary to the vasospasm due to subarachnoid hemorrhage (SAH) is an important cause of morbid-mortality. We propose the use of the PET tracer [ 18 F]Fluoromisonidazole to visualize the hypoxia due to the vasospasm. On the other hand [ 18 F]Fluoromisonidazole synthesis process was optimized, avoiding HPLC purification using SPE cartridges instead, and reducing some synthesis steps. [ 18 F]Fluoromisonidazole in vitro stability was tested for ten hours, and in vivo PET/CT images showed higher cerebral uptake in hemorrhagic animals than in control rats. Highlights: Synthesis of [ 18 F]FMISO with SPE purification completed in 21 min, avoiding HPLC purification. Radiochemical yield of 40% (decay corrected) and radiochemical purity higher than 99%, with good in vitro stability (more than 10 h). [ 18 F]FMISO PET/CT images of hypoxia at striatum in animals with induced subarachnoid hemorrhage.