C—C motif chemokine ligand 2 regulates lps‐induced inflammation and ER stress to enhance proliferation of bovine endometrial epithelial cells. Issue 4 (10th November 2017)

• Record Type: Journal Article
• Authors: Lim, Whasun; Bae, Hyocheol; Bazer, Fuller W.; Kim, Sung‐Man; Song, Gwonhwa
• Is Part Of: Journal of cellular physiology. Volume 233:Issue 4(2018:Apr.)
• Page Start: 3141
• Page End: 3151
• Publication Date: 2017-11-10
• Subjects: CCL2 -- endometrial epithelial cells -- endoplasmic reticulum stress -- immune system; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.26151
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract : Chemokines play an important role in regulating the complex immune system at the maternal‐fetal interface during pregnancy. Among various chemokines, CC motif chemokine ligand 2 (CCL2) plays a role in the recruitment of immune regulatory cells to implantation sites within the endometrium. In cattle, CCL2 is abundantly expressed in the uterine endometrium. However, its intracellular signaling has not been identified. In this study, we examined the effects of CCL2 on bovine endometrial (BEND) cell proliferation. CCL2 stimulated BEND cell proliferation by abundant expression of PCNA, accumulation of cells in the G2/M phase, and activation of the PI3 K/AKT and MAPK signaling pathways. Moreover, CCL2 reduced endoplasmic reticulum stress and restored the inflammation‐induced reduction in BEND cell proliferation by regulating the unfolded protein response genes and cytokines. Collectively, these results demonstrated that CCL2 plays a pivotal role in reproductive tissues and may support maternal‐fetal interface to improve efficiency of pregnancy. Abstract : CCL2 increases proliferation of BEND epithelial cells by increasing PCNA and cyclin D1 expression via the PI3K/AKT and MAPK pathways. CCL2 also suppresses the tunicamycin‐induced ER stress that causes decreased cell proliferation and further reduces the LPS‐induced inflammation that leads to reduction in proliferation of bovine endometrial (BEND) cells.