Frontispiece: Mechanistic Study of the Stereoselective Hydroxylation of [2‐2H1, 3‐2H1]Butanes Catalyzed by Cytochrome P450 BM3 Variants. Issue 11 (21st February 2017)

• Record Type: Journal Article
• Authors: Yang, Chung‐Ling; Lin, Cheng‐Hung; Luo, Wen‐I; Lee, Tsu‐Lin; Ramu, Ravirala; Ng, Kok Yaoh; Tsai, Yi‐Fang; Wei, Guor‐Tzo; Yu, Steve S.‐F.
• Is Part Of: Chemistry. Volume 23:Issue 11(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-02-21
• Subjects: cytochrome P450 -- enzyme catalysis -- heme proteins -- isotope effects -- oxidation -- stereoselectivity; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201781163
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract : Catalytic mechanism of cytochrome P450 A high H/D kinetic isotope effect (KIE) (18–25) is observed from the hydroxylation of tailored di‐deuterated butane substrate, (2 S, 3 S )‐[2‐ 2 H1, 3‐ 2 H1 ]butane, mediated by engineered cytochrome P450 BM3. For the first time, the experimental evidence that supports the participation of quantum tunneling at the secondary C–H bond oxidized by P450 BM3 is demonstrated. This mechanistic revelation provides the basis for the development of biomimetic iron based catalysts for small alkane oxidation or the process for "gas‐to‐liquid" (GTL) conversion. For more information, see the Full Paper by S. Yu et al. onpage 2571 ff.