Determined about sex: Sex-testing in 45 primate species using a 2Y/1X sex-typing assay. (January 2015)

• Record Type: Journal Article
• Authors: Cadamuro, Valérie Choesmel; Bouakaze, Caroline; Croze, Myriam; Schiavinato, Stéphanie; Tonasso, Laure; Gérard, Patrice; Fausser, Jean-Luc; Gibert, Morgane; Dugoujon, Jean-Michel; Braga, José; Balaresque, Patricia
• Is Part Of: Forensic science international. Volume 14(2015:Jan.)
• Page Start: 96
• Page End: 107
• Publication Date: 2015-01
• Subjects: UTX/UTY -- SRY -- Primates -- Multiplex PCR -- Sex-typing; Forensic genetics -- Periodicals; Génétique légale -- Périodiques; Forensic genetics; Electronic journals; Periodicals; 614.1
• DOI: 10.1016/j.fsigen.2014.09.010
• Languages: English
• ISSNs: 1872-4973

Abstract

Abstract: Sex-testing using molecular genetic technique is routinely used in the fields of forensics, population genetics and conservation biology. However, none of the assay used so far allows a non-ambiguous and successful sex determination for human and non-human primate species. The most widely used method, AMELY/X, and its alternatives suffer from a set of drawbacks in humans and can rarely be used in New World primate species. Here, we designed a new sex-typing assay using a multiplexed PCR amplification of UTX and UTY-homologous loci and combined male-specific SRY locus. This method was successfully tested on 1048 samples, including 82 non-human primates from 45 Anthropoidea and Lemuriformes species and 966 human samples from 24 populations (Africans, Europeans, and South Americans). This sex-typing method is applicable across all primate species tested from Hominoidea to Indriidae, and also on various populations with different background origins; it represents a robust and cheap sex-typing assay to be used both by the anthropologist and primatologist communities.