Increased expression of triggering receptor expressed on myeloid cells‐1 in the population with obesity and insulin resistance. Issue 3 (23rd January 2017)

• Record Type: Journal Article
• Authors: Subramanian, Saravanan; Pallati, Pradeep K.; Rai, Vikrant; Sharma, Poonam; Agrawal, Devendra K.; Nandipati, Kalyana C.
• Is Part Of: Obesity. Volume 25:Issue 3(2017)
• Page Start: 527
• Page End: 538
• Publication Date: 2017-01-23
• Subjects: Obesity -- Periodicals; 616.398005
• DOI: 10.1002/oby.21714
• Languages: English
• ISSNs: 1930-7381

Abstract

Abstract : Objective: Triggering receptor expressed on myeloid cells (TREM)−1 has recently been recognized as one of the potent amplifiers of acute and chronic inflammation. However, the exact role of TREM‐1 in regard to insulin insensitivity is unknown. Methods: mRNA transcripts and protein expression of TREM‐1, TREM‐2, and TREM‐1/TREM‐2 ratio were examined in the tissue biopsies (liver, omentum, and subcutaneous fat) and blood samples (neutrophils and monocytes) of subjects with obesity and diabetes (SO + D + ; n = 15), subjects with obesity but not diabetes (SO + D − ; n = 7), and subjects without obesity (BMI < 30) and diabetes (SO − D − ; n = 5). Results: The immunofluorescence and RT‐PCR revealed significant increase in TREM‐1, decrease in TREM‐2, and increase in the TREM1/TREM2 ratio in SO + D + group compared with other groups. Overall, increased liver TREM‐1 expression and soluble‐TREM‐1 were found in SO + D + group compared with SO + D − group (100% vs. 57.14%, r = 0.582; P = 0.023). TREM‐1 was significantly increased in all subjects with obesity and those with HOMA‐IR index of >2. Conclusions: TREM‐1 was found to be significantly higher in tissues biopsies and blood of subjects with obesity. Greater expression and activity of TREM‐1 suggest a possible role in the underlying pathophysiology of obesity and associated comorbidities.