Open the SterivexTM casing: An easy and effective way to improve DNA extraction yields. (31st October 2017)

• Record Type: Journal Article
• Authors: Cruaud, Perrine; Vigneron, Adrien; Fradette, Marie‐Stéphanie; Charette, Steve J.; Rodriguez, Manuel J.; Dorea, Caetano C.; Culley, Alexander I.
• Is Part Of: Limnology and oceanography, methods. Volume 15:Number 12(2017:Dec.)
• Page Start: 1015
• Page End: 1020
• Publication Date: 2017-10-31
• Subjects: Limnology -- Methodology -- Periodicals; Oceanography -- Methodology -- Periodicals; 551.48
• DOI: 10.1002/lom3.10221
• Languages: English
• ISSNs: 1541-5856

Abstract

Abstract: We describe an inexpensive, reliable, and easily executed improvement for the extraction of DNA from Sterivex TM filter units, that involves the separation of the Sterivex TM filter from its casing. Our study demonstrates that our modification of the original extraction protocol significantly increased DNA yields, with an average increase of 4.1‐fold more DNA than with the standard approach. A comparison of the diversity after Illumina MiSeq sequencing of bacterial communities extracted with both the standard approach and the proposed one indicated that our modified protocol has no or little impact on the results. This protocol provides a relatively straight forward means to achieve higher yields of DNA from the extraction of Sterivex TM cartridges without altering the community composition and will likely be of interest to a wide range of scientists that use techniques based on the recovery of DNA from filters.