Online quantification and compound‐specific stable isotopic analysis of black carbon in environmental matrices via liquid chromatography‐isotope ratio mass spectrometry. (16th October 2017) - Wagner, Sasha
Abstract: Black carbon (BC) is derived from the burning of biomass, a considerable portion of organic matter across terrestrial and marine ecosystems, and a major contributor to global carbon cycles. The benzenepolycarboxylic acid method, which converts condensed aromatic BC structures to molecular markers (BPCAs), has been widely adopted for environmental BC assessments. We present a novel analytical method for the online separation, quantification, and determination of compound‐specific stable carbon isotopes of individual BPCAs via high performance liquid chromatography‐stable carbon isotope ratio mass spectrometry (HPLC‐C‐IRMS). To assess δ 13 C values of environmental BC, the HPLC‐C‐IRMS method was applied to a variety of samples (soil, char, aerosol, and dissolved organic matter). The method reliably measured amounts and δ 13 C values of benzenepentacarboxylic acid (B5CA) and mellitic acid (B6CA) over a range of 8.3–130 μ mol‐C of organic matter oxidized. BPCA‐specific δ 13 C values deviated from bulk OC δ 13 C values for different sample types. However, the stable carbon isotopic composition of B5CA and B6CA was found to be generally correlated with that of bulk organic carbon. The stable isotopic compositions of environmental samples covered a wide dynamic range, indicating BC‐specific δ 13 C values will be useful for tracking BC sources and processing. For instance, the BPCAs derived from marine dissolved organic matter (DOM) were enriched in 13 C relative to BPCAs from riverine DOM, suggesting rivers are not the only source of dissolved black carbon to the oceans.