Construction for Δ-12 Fatty Acid Desaturase (FAD2) Silencing to Improve Oil Quality of Jatropha curcas Linn. (2015)

• Record Type: Journal Article
• Authors: Utomo, Condro; Subroto, Andrea Putri; Darmawan, Chris; Setyobudi, Roy Hendroko; Nugroho, Yogo Adhi; Liwang, Tony
• Is Part Of: Energy procedia. Volume 65(2015)
• Page Start: 36
• Page End: 41
• Publication Date: 2015
• Subjects: Δ 12 fatty acid desaturase -- gene silencing -- intron-spliced inverted repeat -- inverted repeat -- Jatropha curcas Linn.; Power resources -- Congresses; Power resources -- Periodicals; Power resources; Conference proceedings; Periodicals; 333.7905
• DOI: 10.1016/j.egypro.2015.01.027
• Languages: English
• ISSNs: 1876-6102

Abstract

Abstract: The predominant fatty acids of jatropha oil are oleic acid (42.02%), linoleic acid (35.38%), palmitic acid (14.54%), and stearic acid (6.3%). Ratio of fatty acids content of jatropha oil, especially for oleic acid (42.02%) compare to linoleic acid (35.38%) is approximately equal. The oxidation stability of jatropha oil showed that methyl oleate is around 2.79 h and methyl linoleate is 0.94 h. Therefore, the presence of methyl linoleate will decrease the value oxidation stability of jatropha oil. The objective of the research was to modify fatty acid composition, especially to increase oleic acid and to reduce linoleic acid content of jatropha oil. To achieve the objective construction of transformation vectors for Δ 12 fatty acid desaturase FAD2 gene silencing was designed. In two transformation vectors, FAD2 gene fragment was constructed in inverted repeat (IR) and intron-spliced inverted repeat (ISIR) orientations to generate hair-pin RNAs (hpRNA).