Molecular and Histochemical Analysis of Jatropha Curcas Linn. Transgenic Using Tolerance Antibiotics Hygromycin (hpt) and β-Glucuronidase (gus-A) Gene. (2015)

• Record Type: Journal Article
• Authors: Maftuchah, ; Zainudin, Agus; Pahlevi, Robby Wahyu; Anggriawan, Arik
• Is Part Of: Energy procedia. Volume 65(2015)
• Page Start: 194
• Page End: 203
• Publication Date: 2015
• Subjects: Gus-A gene -- histochemicals test -- hpt gene -- Jatropha curcas Linn.; Power resources -- Congresses; Power resources -- Periodicals; Power resources; Conference proceedings; Periodicals; 333.7905
• DOI: 10.1016/j.egypro.2015.01.020
• Languages: English
• ISSNs: 1876-6102

Abstract

Abstract: To improve Jatropha curcas's trait, genetic engineering technology genetic engineering development is needed. This study was aimed to analyze transgenic of J. curcas with plasmid vectors pCambia-1304. The study was conducted using primer hpt (forward) 5'-GATGCCTCCGCTCGAAGTAGCG-3', (reverse) 5'-GCATCTCCCGCCGTGCAC-3', gus-A (forward) 5'–GCCATTTGAAGCCGATGTCACGCC-3' and (reverse) 5'-GTATCGGTGTGAGCGTCGCAGAAC-3'. The PCR results elucidated five plants (16.67%) positive hpt gene, 22 plants (77.33%) positive gus -A gene. Histochemical test results showed 16 plants (53.33%) positive hpt test. A total of 24 plants (80%) resulted positive gus -A gene on leaf and 11 plants (36.67%) resulted positive gus -A gene on the root.