Uniform‐sized neurosphere‐mediated motoneuron differentiation in microwell arrays. Issue 24 (6th September 2017)

• Record Type: Journal Article
• Authors: Lee, Jong Min; Moon, Joo Yoon; Shaker, Mohammed R.; Sun, Woong; Chung, Bong Geun
• Is Part Of: Electrophoresis. Volume 38:Issue 24(2017)
• Page Start: 3161
• Page End: 3167
• Publication Date: 2017-09-06
• Subjects: Hydrogel microwell array -- Motoneuron differentiation -- Uniform‐sized neurosphere; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201700118
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract: We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform‐sized neurospheres harvested from microwell arrays were replated into laminin‐coated substrate. In parallel, uniform‐sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three‐dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform‐sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform‐sized neurosphere‐mediated motoneuron differentiation.