VIP induces the decidualization program and conditions the immunoregulation of the implantation process. (15th January 2018)

• Record Type: Journal Article
• Authors: Grasso, Esteban; Gori, Soledad; Paparini, Daniel; Soczewski, Elizabeth; Fernández, Laura; Gallino, Lucila; Salamone, Gabriela; Martinez, Gustavo; Irigoyen, Marcela; Ruhlmann, Claudio; Pérez Leirós, Claudia; Ramhorst, Rosanna
• Is Part Of: Molecular and cellular endocrinology. Volume 460(2018)
• Page Start: 63
• Page End: 72
• Publication Date: 2018-01-15
• Subjects: VIP -- Implantation -- Immunoregulation -- Decidualization; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2017.07.006
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: The decidualization process involves phenotype and functional changes on endometrial cells and the modulation of mediators with immunoregulatory properties as the vasoactive intestinal peptide (VIP). We investigate VIP contribution to the decidualization program and to immunoregulation throughout the human embryo implantation process. The decidualization of Human endometrial stromal cell line (HESC) with Medroxyprogesterone-dibutyryl-cAMP increased VIP/VPAC-receptors system. In fact, VIP could induce decidualization increasing differentiation markers (IGFBP1, PRL, KLF13/KLF9 ratio, CXCL12, CXCL8 and CCL2) and allowing Blastocyst-like spheroids (BLS) invasion in an in vitro model of embryo implantation. Focus on the tolerogenic effects, decidualized cells induced a semi-mature profile on maternal dendritic cells; restrained CD4 + cells recruitment while increased regulatory T-cells recruitment. Interestingly, the human blastocyst conditioned media from developmentally impaired embryos diminished the invasion and T-regulatory cells recruitment in these settings. These evidences suggest that VIP contributes to the implantation process inducing decidualization, allowing BLS invasion and favoring a tolerogenic micro-environment. Highlights: VIP induced decidualization in HESC cells along with increased decidualization markers. Blastocyst-like spheroids were able to invade HESC decidualized with VIP or MPA + dbcAMP. Decidualized HESC conditioned media induced a semi-mature profile on dendritic cells. Decidualized HESC conditioned media restrained CD4 + cells recruitment while increased CD4+Foxp3+ recruitment. Human blastocyst conditioned media from developmentally impaired embryos diminished the invasion and Treg recruitment.