Surface Curvature Differentially Regulates Stem Cell Migration and Differentiation via Altered Attachment Morphology and Nuclear Deformation. Issue 2 (20th December 2016)

• Record Type: Journal Article
• Authors: Werner, Maike; Blanquer, Sébastien B. G.; Haimi, Suvi P.; Korus, Gabriela; Dunlop, John W. C.; Duda, Georg N.; Grijpma, Dirk. W.; Petersen, Ansgar
• Is Part Of: Advanced science. Volume 4:Issue 2(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-12-20
• Subjects: 3D surface curvature -- cell migration -- nuclear mechanics -- osteogenic differentiation -- stereolithography; Science -- Periodicals; 505
• DOI: 10.1002/advs.201600347
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract : Signals from the microenvironment around a cell are known to influence cell behavior. Material properties, such as biochemical composition and substrate stiffness, are today accepted as significant regulators of stem cell fate. The knowledge of how cell behavior is influenced by 3D geometric cues is, however, strongly limited despite its potential relevance for the understanding of tissue regenerative processes and the design of biomaterials. Here, the role of surface curvature on the migratory and differentiation behavior of human mesenchymal stem cells (hMSCs) has been investigated on 3D surfaces with well‐defined geometric features produced by stereolithography. Time lapse microscopy reveals a significant increase of cell migration speed on concave spherical compared to convex spherical structures and flat surfaces resulting from an upward‐lift of the cell body due to cytoskeletal forces. On convex surfaces, cytoskeletal forces lead to substantial nuclear deformation, increase lamin‐A levels and promote osteogenic differentiation. The findings of this study demonstrate a so far missing link between 3D surface curvature and hMSC behavior. This will not only help to better understand the role of extracellular matrix architecture in health and disease but also give new insights in how 3D geometries can be used as a cell‐instructive material parameter in the field of biomaterial‐guided tissue regeneration. Abstract : The influence of 3D surface curvature on stem cell migration and differentiation is reported. Concave surfaces enable the cell body to lift off the surface resulting in increased motility while on convex surfaces, cytoskeletal tension induces nuclear deformation and enhances osteogenic differentiation. The study reveals curvature‐sensation of stem cells with relevance for biomaterial design and tissue regeneration.