Generation and characterization of new alleles of quiver (qvr) that encodes an extracellular modulator of the Shaker potassium channel. (2nd October 2017)

• Record Type: Journal Article
• Authors: Ruan, Hongyu; Ueda, Atsushi; Xing, Xiaomin; Wan, Xuxuan; Strub, Benjamin; Mukai, Spencer; Certel, Kaan; Green, David; Belozerov, Kyle; Yao, Wei-Dong; Johnson, Wayne; Jung-Ching Lin, Jim; Hilliker, Arthur J.; Wu, Chun-Fang
• Is Part Of: Journal of neurogenetics. Volume 31:Number 4(2017)
• Page Start: 325
• Page End: 336
• Publication Date: 2017-10-02
• Subjects: qvr/sss -- Shaker -- sleepless -- RNA editing -- Ly-6/neurotoxin superfamily -- synaptic plasticity -- potassium current inactivation -- ether-induced leg shaking; Neurogenetics -- Periodicals; 616.80442
• DOI: 10.1080/01677063.2017.1393076
• Languages: English
• ISSNs: 0167-7063

Abstract

Abstract: Our earlier genetic screen uncovered a paraquat-sensitive leg-shaking mutant quiver 1 ( qvr 1 ), whose gene product interacts with the Shaker ( Sh ) K + channel. We also mapped the qvr locus to EY04063 and noticed altered day–night activity patterns in these mutants. Such circadian behavioral defects were independently reported by another group, who employed the qvr 1 allele we supplied them, and attributed the extreme restless phenotype of EY04063 to the qvr gene. However, their report adopted a new noncanonical gene name sleepless ( sss ) for qvr . In addition to qvr 1 and qvr EY, our continuous effort since the early 2000s generated a number of novel recessive qvr alleles, including ethyl methanesulfonate (EMS)-induced mutations qvr 2 and qvr 3, and P-element excision lines qvr ip6 (imprecise jumpout), qvr rv7, and qvr rv9 (revertants) derived from qvr EY . Distinct from the original intron-located qvr 1 allele that generates abnormal-sized mRNAs, qvr 2, and qvr 3 had their lesion sites in exons 6 and 7, respectively, producing nearly normal-sized mRNA products. A set of RNA-editing sites are nearby the lesion sites of qvr 3 and qvr EY on exon 7. Except for the revertants, all qvr alleles display a clear ether-induced leg-shaking phenotype just like Sh, and weakened climbing abilities to varying degrees. Unlike Sh, all shaking qvr alleles (except for qvr f01257 ) displayed a unique activity-dependent enhancement in excitatory junction potentials (EJPs) at larval neuromuscular junctions (NMJs) at very low stimulus frequencies, with qvr EY displaying the largest EJP and more significant NMJ overgrowth than other alleles. Our detailed characterization of a collection of qvr alleles helps to establish links between novel molecular lesions and different behavioral and physiological consequences, revealing how modifications of the qvr gene lead to a wide spectrum of phenotypes, including neuromuscular hyperexcitability, defective motor ability and activity-rest cycles.