Biodegradation of benzo[a]pyrene by bacterial consortium isolated from mangrove sediment. Issue 4 (16th February 2018)

• Record Type: Journal Article
• Authors: Aziz, A.; Agamuthu, P.; Alaribe, F.O.; Fauziah, S.H.
• Is Part Of: Environmental technology. Volume 39:Issue 4(2018)
• Page Start: 527
• Page End: 535
• Publication Date: 2018-02-16
• Subjects: Benzo[a]pyrene -- biodegradation -- co-metabolism -- bacterial consortium; Environmental engineering -- Periodicals; Environmental protection -- Periodicals; 628.05
• DOI: 10.1080/09593330.2017.1305455
• Languages: English
• ISSNs: 0959-3330

Abstract

ABSTRACT: Benzo[a]pyrene is a high-molecular-weight polycyclic aromatic hydrocarbon highly recalcitrant in nature and thus harms the ecosystem and/or human health. Therefore, its removal from the marine environment is crucial. This research focuses on benzo[a]pyrene degradation by using enriched bacterial isolates in consortium under saline conditions. Bacterial isolates capable of using benzo[a]pyrene as sole source of carbon and energy were isolated from enriched mangrove sediment. These isolates were identified as Ochrobactrum anthropi, Stenotrophomonas acidaminiphila, and Aeromonas salmonicida ss salmonicida . Isolated O . anthropi and S . acidaminiphila degraded 26% and 20%, respectively, of an initial benzo[a]pyrene concentration of 20 mg/L after 8 days of incubation in seawater (28 ppm of NaCl). Meanwhile, the bacterial consortium decomposed 41% of an initial 50 mg/L benzo[a]pyrene concentration after 8 days of incubation in seawater (28 ppm of NaCl). The degradation efficiency of benzo[a]pyrene increased to 54%, when phenanthrene was supplemented as a co-metabolic substrate. The order of biodegradation rate by temperature was 30°C > 25°C > 35°C. Our results suggest that co-metabolism by the consortium could be a promising biodegradation strategy for benzo[a]pyrene in seawater.