A simple method for purification of bromelain in a thermosensitive triblock copolymer-based protection system and recycling of phase components. Issue 4 (4th March 2018)

• Record Type: Journal Article
• Authors: Han, Juan; Cai, Yunfeng; Xie, Xueqiao; Wang, Yun; Wang, Li; Li, Cheng; Rao, Wenwei; Ni, Liang
• Is Part Of: Separation science and technology. Volume 53:Issue 4(2018)
• Page Start: 636
• Page End: 644
• Publication Date: 2018-03-04
• Subjects: Aqueous two-phase systems -- thermoseparating polymers -- recycle -- extraction; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2017.1398757
• Languages: English
• ISSNs: 0149-6395

Abstract

ABSTRACT: In the work, we chose stem bromelain as a model to investigate the storage and purification of bromelain from pineapple peel. Extraction of bromelain from pineapple peel using a two-stage aqueous two-phase extraction system composed of a thermoseparating copolymer EOPOEO and K2 HPO4 . Bromelain predominantly partitioned to the EOPOEO-rich phase and then re-extract to the top dilute phase. The recovery of enzyme activity (68.6%) and purification factor (6.53) were determined under optimum conditions. The EOPOEO-rich phase and salt were recycled, and the recovery of enzyme activity could reach up to 60%. This method has been proved to obtain highly purified and stable bromelain.