Regulation function of MMP-1 downregulated by siRNA on migration of heat-denatured dermal fibroblasts. Issue 6 (2nd November 2017)

• Record Type: Journal Article
• Authors: He, Xianghui; Dai, Jinhua; Fan, Youfen; Zhang, Chun; Zhao, Xihong
• Is Part Of: Bioengineered. Volume 8:Issue 6(2017)
• Page Start: 686
• Page End: 692
• Publication Date: 2017-11-02
• Subjects: heat-denatured dermal fibroblast -- MMP-1 -- migration -- siRNA -- TIMP; Biomedical engineering -- Periodicals; Biotechnology -- Periodicals; Microbiology -- Periodicals; 660.6
• DOI: 10.1080/21655979.2016.1267885
• Languages: English
• ISSNs: 2165-5987

Abstract

ABSTRACT: Cutaneous wound healing is a complex physiological process that requires the efforts of various cell types and signaling pathways and often results in thickened collagen-enriched healed tissue called a scar. Therefore, the identification of the mechanism of cutaneous wound healing is necessary and has great value in providing better treatment. Here, we demonstrated that MMP-1 inhibition could promote cell proliferation in dermal fibroblasts via the MTT assay. Meanwhile, we investigated cell migration by flow cytometry and tested type I collagenase activity. We found that MMP-1 inhibition promoted cell proliferation and inhibited cell migration and type I collagenase activity. In conclusion, our study demonstrated that MMP-1 might be a potential therapeutic target in cutaneous wound healing.