Cover Picture: Debugging Eukaryotic Genetic Code Expansion for Site‐Specific Click‐PAINT Super‐Resolution Microscopy (Angew. Chem. Int. Ed. 52/2016). Issue 52 (1st December 2016)

• Record Type: Journal Article
• Authors: Nikić, Ivana; Estrada Girona, Gemma; Kang, Jun Hee; Paci, Giulia; Mikhaleva, Sofya; Koehler, Christine; Shymanska, Nataliia V.; Ventura Santos, Camilla; Spitz, Daniel; Lemke, Edward A.
• Is Part Of: Angewandte Chemie international edition. Volume 55:Issue 52(2016)
• Page Start: 15931
• Page End: 15931
• Publication Date: 2016-12-01
• Subjects: click chemistry -- genetic code expansion -- PAINT -- protein labeling -- super-resolution microscopy; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201611395
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract : Cytoplasmic re‐targeting of the pyrrolysine‐based genetic code expansion machinery by using a nuclear export signal (NES) substantially increases the site‐specific incorporation of clickable amino acids (green) into proteins in mammalian cells, as shown by E. A. Lemke and co‐workers in their Communication onpage 16172 ff. These clickable amino acids can then be modified with annealing sites for small DNA imaging strands (red) to enable high‐contrast Click‐PAINT super‐resolution microscopy.