Mcp, aer, cheB, and cheV contribute to the regulation of Vibrio alginolyticus (ND‐01) adhesion under gradients of environmental factors. Issue 6 (25th July 2017) - Huang, Lixing
Abstract: Adhesion is a key virulence factor of pathogens and can be affected by the environment. Our previously research with RNA‐seq indicated that mcp, aer, cheB, and cheV might play roles in the regulation of adhesion in Vibrio alginolyticus (ND‐01). In order to determine whether and how environmental factors affect adhesion through these genes, gene silencing was performed followed by quantitative real‐time PCR (qRT‐PCR), RNAi, transmission electron microscopy, and adhesion, capillary, and motility assays to verify how these genes influence adhesion. Silencing these genes led to deficiencies in adhesion, chemotaxis, flagellar assembly, and motility. The expression levels of cheA, cheW, and cheY, which are important genes closely related to the functions of mcp, aer, cheV, and cheB, were significantly downregulated in all of the RNAi groups. The expression of mcp, aer, cheV, and cheB under different gradients of temperature, pH, and salinity and after starvation for various durations was also detected, which showed that these genes were sensitive to certain environmental stresses, particularly pH and starvation. Our results indicated that mcp, aer, cheB, and cheV : (1) are necessary for ND‐01 adhesion; (2) play key roles in the bacterial chemotaxis pathway by controlling the expression of downstream genes; (3) might affect adhesion by impacting motility, though motility is not the only route through which adhesion is affected; and (4) contribute to the regulation of ND‐01 adhesion in natural environments with different temperatures, pH levels, and salinities as well as after various starvation periods. Abstract : RNAi of mcp, aer, cheB, and cheV result in the decrease in Vibrio alginolyticus adhesion. Environmental factors affect the gene expression of mcp, aer, cheB, and cheV.