Architecture of the yeast Elongator complex. (14th December 2016)

• Record Type: Journal Article
• Authors: Dauden, Maria I; Kosinski, Jan; Kolaj‐Robin, Olga; Desfosses, Ambroise; Ori, Alessandro; Faux, Celine; Hoffmann, Niklas A; Onuma, Osita F; Breunig, Karin D; Beck, Martin; Sachse, Carsten; Séraphin, Bertrand; Glatt, Sebastian; Müller, Christoph W
• Is Part Of: EMBO reports. Volume 18:Number 2(2017)
• Page Start: 264
• Page End: 279
• Publication Date: 2016-12-14
• Subjects: electron microscopy -- Elongator -- Saccharomyces cerevisiae -- tRNA modification -- yeast; Molecular biology -- Periodicals; Molecular Biology -- Periodicals; Molecular biology; Periodicals; 572.8
• DOI: 10.15252/embr.201643353
• Languages: English
• ISSNs: 1469-221X

Abstract

Abstract: The highly conserved eukaryotic Elongator complex performs specific chemical modifications on wobble base uridines of tRNAs, which are essential for proteome stability and homeostasis. The complex is formed by six individual subunits (Elp1‐6) that are all equally important for its tRNA modification activity. However, its overall architecture and the detailed reaction mechanism remain elusive. Here, we report the structures of the fully assembled yeast Elongator and the Elp123 sub‐complex solved by an integrative structure determination approach showing that two copies of the Elp1, Elp2, and Elp3 subunits form a two‐lobed scaffold, which binds Elp456 asymmetrically. Our topological models are consistent with previous studies on individual subunits and further validated by complementary biochemical analyses. Our study provides a structural framework on how the tRNA modification activity is carried out by Elongator. Synopsis: The conserved Elongator complex specifically modifies tRNAs. An integrative modelling approach using data from negative‐stain EM and crosslinking mass spectrometry is used to obtain an architectural model of the fully assembled Elongator complex. Elp456 assembles asymmetrically on the Elp123 sub‐complex to form holoElongator. A dense network of interactions connects all six Elongator subunits. The enzymatically active Elp3 subunits are located in the center of this network. Abstract : The conserved Elongator complex specifically modifies tRNAs. An integrative modelling approach using data from negative‐stain EM and crosslinking mass spectrometry is used to obtain an architectural model of the fully assembled Elongator complex.