Transcriptome analysis reveals novel insights into the response to Pb exposure in Phanerochaete chrysosporium. (March 2018)

• Record Type: Journal Article
• Authors: Huang, Chao; Wang, Rongzhong; Zeng, Guangming; Huang, Danlian; Lai, Cui; Zhang, Jiachao; Xiao, Zhihua; Wan, Jia; Xu, Piao; Gong, Xiaomin; Xue, Wenjing; Ren, Xiaoya
• Is Part Of: Chemosphere. Volume 194(2018)
• Page Start: 657
• Page End: 665
• Publication Date: 2018-03
• Subjects: Phanerochaete chrysosporium -- Pb -- cDNA-AFLP -- TDFs -- Gene expression; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2017.12.046
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Metals released into the environment continue to be of concern for human health. Using white-rot fungi as biosorbents for heavy metals removal is an attractive alternative owing to its good performance and low cost. However, the molecular mechanism underlying heavy metal tolerance in white-rot fungi has not yet been fully elucidated. This study identified and analyzed the lead (Pb)-induced transcriptional changes in Phanerochaete chrysosporium, a well-known heavy metal hyperaccumulating white-rot fungus. The results confirmed its outstanding ability in Pb tolerance and effective defense system. By comparative analysis of gene expression profiles obtained from cDNA-amplified fragment length polymorphism (cDNA-AFLP), we isolated 43 transcript-derived fragments (TDFs) differentially regulated by Pb exposure in P. chrysosporium, and 23 TDFs presented significant similarities to genes encoding known or putative proteins which belong to different functional categories involving ion binding, energy and carbohydrate metabolism, and signal transduction. The detailed characterization of these Pb-responsive genes was presented and the expression patterns of some interesting genes were validated by quantitative RT-PCR. This work provides the first evidence of Pb-responsive genes along with their putatively functional annotations in P. chrysosporium, which may help to understand the mechanism underlying heavy metal accumulation and tolerance in P. chrysosporium . Graphical abstract: Highlights: Pb affected fungal growth and Pb accumulation in a dose- and time-dependent manner. cDNA-AFLP analysis revealed the Pb-responsive genes in P. chrysosporium. Genes involved in ion binding, energy and signal transduction were altered. qRT-PCR analysis of six Pb-responsive genes validated the cDNA-AFLP result.