Characterization and expression analysis of a chitinase gene (PmChi-5) from black tiger shrimp (Penaeus monodon) under pathogens infection and ambient ammonia-N stress. Issue 72 (January 2018)

• Record Type: Journal Article
• Authors: Zhou, Falin; Zhou, Kaimin; Huang, Jianhua; Yang, Qibin; Jiang, Song; Qiu, Lihua; Yang, Lishi; Jiang, Shigui
• Is Part Of: Fish & shellfish immunology. Issue 72(2018)
• Page Start: 117
• Page End: 123
• Publication Date: 2018-01
• Subjects: Penaeus monodon -- Chitinase -- Streptococcus agalactiae -- Vibrio harveyi -- Ammonia nitrogen stress; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.10.051
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Chitinases are crucial enzymes for crustaceans. Previous researches had already revealed that chitinases play important roles in digestion, molting and defense against viruses. In the present study, a chitinase cDNA was identified from black tiger shrimp ( Penaeus monodon ) and designated as PmChi-5 . The full-length PmChi-5 cDNA was 2860 bp in size, containing an open reading frame (ORF) of 1731 bp that encoded a protein of 576 amino acids with a deduced molecular weight of 64.8 kDa. Expression of the PmChi-5 mRNA was ubiquitously detected in all selected tissues, with the highest level in the gill and hepatopancreas. PmChi - 5 was expressed throughout the whole larvae stages, and the highest level at Mysis3 stage, which indicated that PmChi-5 may be involved in larval metamorphosis. After challenged with Streptococcus agalactiae and Vibrio harveyi, the transcripts of PmChi-5 were found to be up-regulated significantly both in hepatopancreas and gill. Besides, the ammonia nitrogen stress treatment was also carried out, PmChi-5 transcripts were significantly changed in hepatopancreas and gill. The results showed that PmChi-5 may be involved in molting, larval metamorphosis, the immune defenses to pathogens infection and ammonia-N stress. Highlights: PmChi-5 gene was identified and characterized in Penaeus monodon. The expression of the PmChi-5 mRNA was ubiquitously detected in all selected tissues. PmChi - 5 was expressed throughout the whole larvae stages, while the highest level was detected in the Mysis3 stage. The expression pattern of PmChi - 5 during the molt cycle was investigated using qRT-PCR. The expression patterns of PmChi - 5 post bacterial infection and ammonia nitrogen stress were studied.