Involvement of clip-domain serine protease in the anti-Vibrio immune response of abalone (Haliotis discus hannai)–Molecular cloning, characterization and functional analysis. Issue 72 (January 2018)

• Record Type: Journal Article
• Authors: Hu, Jian-Jian; Chen, Yu-Lei; Duan, Xue-Kun; Jin, Teng-Chuan; Li, Yue; Zhang, Ling-Jing; Liu, Guang-Ming; Cao, Min-Jie
• Is Part Of: Fish & shellfish immunology. Issue 72(2018)
• Page Start: 210
• Page End: 219
• Publication Date: 2018-01
• Subjects: Clip-domain serine protease -- Haliotis discus hannai -- Vibrio parahemolyticus -- Innate immunity -- Apoptosis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.10.062
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Vibrio parahemolyticus ( V. parahemolyticus ) is a major pathogen for abalone, an important economical shellfish in coastal area of China. There is little known about the abalone innate immune system against pathogen infection. Clip-domain serine proteases (cSPs) are increasingly recognized to play important roles in host immune defense in invertebrates. In this study, we cloned a cSP (Hdh-cSP) from abalone ( Haliotis discus hannai ). We found out that Hdh-cSP was widely expressed in multiple tissues of abalone, with highest level in the immune-like organ, hepatopancreas. V. parahemolyticus infection induced significantly elevated expression of Hdh-cSP in addition to better-characterized innate immune component genes including Rel/NF-κB, allograft inflammatory factor (ALInFa), macrophage expressed protein (MEP) and caspase-8. Importantly, the silencing of Hdh-cSP reduced the expression of these genes, suggesting that Hdh-cSP was an upstream regulatory factor in V. parahemolyticus infection. Further analysis showed that apoptosis of hemocytes was inhibited when the transcription of Hdh-cSP was knocked down, suggesting that Hdh-cSP participated in cell apoptosis by regulation of caspase 8 expression in V. parahemolyticus infection. Therefore, our study established an important role of cSP in the innate immunity against V. parahemolyticus infection in abalone. Highlights: A clip-domain serine protease (Hdh-cSP) was identified in Haliotis discus hannai. Induced expression of Hdh-cSP was observed after Vibrio parahemolyticus infection. Hdh-cSP regulated the expression of some well-known immune factors. Hdh-cSP was involved in the regulation of hemocytes apoptosis in abalone.