Assessment of genotoxic effects of flumorph by the comet assay in mice organs. (March 2014)

• Record Type: Journal Article
• Authors: Zhang, T; Zhao, Q; Zhang, Y; Ning, J
• Is Part Of: Human & experimental toxicology. Volume 33:Number 3(2014:Mar.)
• Page Start: 224
• Page End: 229
• Publication Date: 2014-03
• Subjects: flumorph -- alkaline comet assay -- genotoxicity -- DNA damage; Toxicology -- Periodicals; 615.9
• DOI: 10.1177/0960327111417268
• Languages: English
• ISSNs: 0960-3271

Abstract

The present study investigated the genotoxic effects of flumorph in various organs (brain, liver, spleen, kidney and sperm) of mice. The DNA damage, measured as comet tail length (µm), was determined using the alkaline comet assay. The comet assay is a sensitive assay for the detection of genotoxicity caused by flumorph using mice as a model. Statistically significant increases in comet assay for both dose-dependent and duration-dependent DNA damage were observed in all the organs assessed. The organs exhibited the maximum DNA damage in 96 h at 54 mg/kg body weight. Brain showed maximum DNA damage followed by spleen > kidney > liver > sperm. Our data demonstrated that flumorph had induced systemic genotoxicity in mammals as it caused DNA damage in all tested vital organs, especially in brain and spleen.