Characterisation of ZBTB46 and DC-SCRIPT/ZNF366 in rainbow trout, transcription factors potentially involved in dendritic cell maturation and activation in fish. (March 2018)

• Record Type: Journal Article
• Authors: Wang, Jun; Wang, Tiehui; Benedicenti, Ottavia; Collins, Catherine; Wang, Kaiyu; Secombes, Christopher J.; Zou, Jun
• Is Part Of: Developmental and comparative immunology. Volume 80(2018)
• Page Start: 2
• Page End: 14
• Publication Date: 2018-03
• Subjects: ZBTB46 -- DC-SCRIPT/ZNF366 -- Dendritic cell -- Transcription factor -- Gene expression -- Interferon; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2016.11.007
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: ZBTB46 and DC-SCRIPT/ZNF366 are two zinc finger transcription factors that play important roles in regulating differentiation of dendritic cells in mammals. In this study, the ZBTB46 and DC-SCRIPT/ZNF366 homologues were identified in rainbow trout Oncorhynchus mykiss and their expression analysed in vivo and in vitro . As transcription factors, they are well conserved in sequence, genomic organisation and gene synteny. Their expression was differentially modulated by bacterial and viral PAMPs in the monocyte/macrophage-like cell line RTS-11, in primary head kidney (HK) macrophages, and in HK macrophages cultured with IL-4/13A. In the RTS-11 cells and primary HK macrophages, all the ZBTB46 and DC-SCRIPT/ZNF366 homologues were down-regulated by interferon gamma (type II IFN) but unaffected by IFN2 (type I IFN), administered as recombinant proteins to cell cultures. In fish gills, infection with amoebae ( Paramoebae perurans ) resulted in reduction of ZBTB46 and DC-SCRIPT/ZNF366 expression in Atlantic salmon Salmo salar, whilst infection with Yersinia ruckeri induced gene expression in rainbow trout. Highlights: The homologues of zinc finger proteins, ZBTB46 and DC-SCRIPT/ZNF366, were identified in rainbow trout. The expression of ZBTB46 and DC-SCRIPT/ZNF366 was differentially modulated by bacterial and viral PAMPs. ZBTB46 and DC-SCRIPT/ZNF366 expression was down-regulated by IFNγ in RTS-11 cells and primary head kidney macrophages. Infection with P. perurans resulted in reduction of ZBTB46 and DC-SCRIPT/ZNF366 expression in gills of Atlantic salmon. Infection with Y. ruckeri induced expression in gills but reduced expression in spleen of rainbow trout.