Universal biomarker analysis using GC×GC with dual FID and ToF-MS (EI/FI) detection. (January 2018)

• Record Type: Journal Article
• Authors: Walters, Clifford C.; Wang, Frank C.; Higgins, Meytal B.; Madincea, Marlene E.
• Is Part Of: Organic geochemistry. Volume 115(2018)
• Page Start: 57
• Page End: 66
• Publication Date: 2018-01
• Subjects: Organic geochemistry -- Periodicals; Biogeochemistry -- Periodicals; Géochimie organique -- Périodiques; 553.205
• DOI: 10.1016/j.orggeochem.2017.10.003
• Languages: English
• ISSNs: 0146-6380

Abstract

Graphical abstract: Highlights: GC×GC with dual FID and ToF-MS (EI/FI) detection provides superior biomarker analyses. Non-targeted, pseudo parent-daughter mass spectra are available for all components. Capillary flow technology provides a consistent split between FID and MS detectors. FID response can be used for absolute quantification without response factors. Abstract: Quantitative and qualitative biomarker analysis using GC-MS or GC-MS/MS is limited by chromatographic separation and variable detector response. Here, we present a GC×GC method that provides absolute quantification and non-targeted pseudo MS-MS for all resolved saturated hydrocarbon components in petroleum. Two innovations make this possible: Agilent capillary flow technology (CFT) and the JEOL JMS-T100GCV 4G ToF-MS capable of electron impact (EI) and field ionization (FI) of the GC×GC effluent. Two sequential chromatograms run using EI- and FI-MS are combined, yielding pseudo parent-daughter spectra. The effluent is split at a constant ratio allowing for easy quantitation by FID and eliminating the need for determining MS-response factors. We demonstrate the technique as offering a means for universal biomarker analysis of saturated hydrocarbon fractions.