Dual targeting c-met and VEGFR2 in osteoblasts suppresses growth and osteolysis of prostate cancer bone metastasis. (1st February 2018)

• Record Type: Journal Article
• Authors: Lee, Changki; Whang, Young Mi; Campbell, Preston; Mulcrone, Patrick L.; Elefteriou, Florent; Cho, Sun Wook; Park, Serk In
• Is Part Of: Cancer letters. Volume 414(2018)
• Page Start: 205
• Page End: 213
• Publication Date: 2018-02-01
• Subjects: Prostate cancer -- Bone metastasis -- Osteoblasts -- c-Met -- VEGFR2; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2017.11.016
• Languages: English
• ISSNs: 0304-3835

Abstract

Abstract: Prostate cancer characteristically induces osteoblastic bone metastasis, for which no therapies are available. A dual kinase inhibitor of c-Met and VEGFR-2 (cabozantinib) was shown to reduce prostate cancer growth in bone, with evidence for suppressing osteoblastic activity. However, c-Met and VEGFR2 signaling in osteoblasts in the context of bone metastasis remain unclear. Here we show using cultured osteoblasts that hepatocyte growth factor (HGF) and VEGF-A increased receptor activator of NFκB ligand (RANKL) and M-CSF, two essential factors for osteoclastogenesis. Insulin-like growth factor-1 (IGF1) also increased RANKL and M-CSF via c-Met transactivation. The conditioned media from IGF1-, HGF-, or VEGFA-treated osteoblasts promoted osteoclastogenesis that was reversed by inhibiting c-Met and/or VEGFR2 in osteoblasts. In vivo experiments used cabozantinib-resistant prostate cancer cells (PC-3 and C4-2B) to test the effects of c-Met/VEGFR2 inhibition specifically in osteoblasts. Cabozantinib (60 mg/kg, 3 weeks) suppressed tumor growth in bone and reduced expression of RANKL and M-CSF and subsequent tumor-induced osteolysis. Collectively, inhibition of c-Met and VEGFR2 in osteoblasts reduced RANKL and M-CSF expression, and associated with reduction of tumor-induced osteolysis, suggesting that c-Met and VEGFR2 are promising therapeutic targets in bone metastasis. Highlights: HGF, VEGF-A and IGF1 increase M-CSF and RANKL in osteoblasts of the bone metastasis. HGF, VEGF-A and IGF1 induce osteoclastogenesis via activation of osteoblasts. A c-Met/VEGFR2 inhibitor suppresses osteoblasts and subsequent osteoclastogenesis. Targeting c-Met and VEGFR2 in osteoblast suppresses prostate cancer bone metastasis. Osteoblasts are promising stromal cell target for the treatment of bone metastasis.