Ischemic postconditioning alters the gene expression pattern of the ischemic heart. (February 2014)

• Record Type: Journal Article
• Authors: Csonka, Csaba; Szűcs, Gergő; Varga-Orvos, Zoltán; Bencsik, Péter; Csont, Tamás; Zvara, Ágnes; Puskás, László G; Ferdinandy, Péter
• Is Part Of: Experimental biology and medicine. Volume 239:Number 2(2014:Feb.)
• Page Start: 141
• Page End: 150
• Publication Date: 2014-02
• Subjects: Postconditioning -- microarray -- gene ontology -- heart -- rat; Physiology -- Periodicals; Biology, Experimental -- Periodicals; Medicine, Experimental -- Periodicals; 610.72
• DOI: 10.1177/1535370213511017
• Languages: English
• ISSNs: 1535-3702

Abstract

To profile changes in gene expression in response to ischemic postconditioning, isolated rat hearts were subjected to 30 min of regional ischemia followed by 120 min of reperfusion with or without postconditioning. At the end of reperfusion, cardiac RNA was assayed by DNA microarrays (31, 000 format), verified by quantitative real-time polymerase chain reaction (QRT-PCR). Postconditioning significantly up-regulated 50 genes and down-regulated 58 different genes, including pyruvate dehydrogenase, 60 kDa heat shock protein 1, lipoprotein lipase, gamma-sarcoglycan, and phospholipase C. Gene ontology analysis revealed that most of the altered genes belong to the cellular metabolic processes cluster. Many of the genes have not previously been suspected to be involved in the mechanism of postconditioning.