Sample preparation for polar metabolites in bioanalysis. Issue 1 (7th December 2017)

• Record Type: Journal Article
• Authors: Drouin, Nicolas; Rudaz, Serge; Schappler, Julie
• Is Part Of: Analyst. Volume 143:Issue 1(2018)
• Page Start: 16
• Page End: 20
• Publication Date: 2017-12-07
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c7an01333g
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Sample preparation is a primary step of any bioanalytical workflow, especially in metabolomics where maximum information has to be obtained without spoiling the analytical instrument. The sample extraction of polar metabolites is still challenging but strategies exist to enable the phase transfer of hydrophilic metabolites from the biological phase to a clean interference-free phase. Abstract : Sample preparation is a primary step of any bioanalytical workflow, especially in metabolomics analysis where maximum information has to be obtained without spoiling the analytical instrument. Because of their biological implication, highly polar metabolites, such as amino acids, nucleobases, and catecholamines seem to attract growing interest in the field of comprehensive metabolomics analysis although their extraction from the matrix remains a real challenge. In this paper, we discuss about the actual practice and issues of hydrophilic metabolites' extraction, including new solutions and perspectives to improve their phase transfer from a complex biological sample to a clean extract prior to analysis.