A mitochondrial-targeting near-infrared fluorescent probe for bioimaging and evaluating endogenous superoxide anion changes during ischemia/reperfusion injury. (February 2018)

• Record Type: Journal Article
• Authors: Han, Xiaoyue; Wang, Rui; Song, Xinyu; Yu, Fabiao; Lv, Changjun; Chen, Lingxin
• Is Part Of: Biomaterials. Volume 156(2018)
• Page Start: 134
• Page End: 146
• Publication Date: 2018-02
• Subjects: Near-infrared fluorescent probes -- Ratiometric bioimaging -- Superoxide anion -- Mitochondrial localization -- Ischemia/reperfusion injury; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2017.11.039
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: The outburst of superoxide anion (O2 − ) in mitochondrial during ischemia/reperfusion (I/R) process will cause a series of oxidative damage including polarity loss of mitochondrial membrane potential, overload of secondary cellular calcium, and cascade apoptosis. To monitor the O2 − level fluctuations as well as to evaluate the relationship between O2 − concentration and the degree of cell apoptosis during I/R process, we propose a ratiometric near-infrared mitochondrial targeting fluorescent probeMito-Cy-Tfs for the detection of level changes of O2 − in cells and in vivo . The probeMito-Cy-Tfs is composed of three moieties: near-infrared heptamethine cyanine as fluorescence signal transducer, trifluoromethanesulfonamide as fluorescence modulator, and lipophilic triphenylphosphonium cation as mitochondrial guider. The probe can well locate in mitochondria and respond the concentration changes of endogenous O2 − selectively and sensitively. The probe has been successfully utilized to image the endogenous O2 − fluctuations in four kinds of cell I/R models (glucose deprivation/reperfusion, serum deprivation/reperfusion, oxygen deprivation/reperfusion and glucose-serum-oxygen deprivation/reperfusion). The probe also exhibits deep tissue penetration for real-time imaging of O2 − concentration in liver of I/R mice model. We confirm that the adoption of ischemic preconditioning (IPC) and postconditioning (IPTC) can protect liver from I/R injury. The probe can be employed to accurately indicate and evaluate the mutual relationship between the levels of O2 − and the degrees of organ damage during I/R, IPC and IPTC processes. The above applications make our new probe a potential candidate for the clinical surgery assessment. Highlights: A mitochondrial-targeting near-infrared ratiometric fluorescent probe for bioimaging during ischemia/reperfusion (I/R) injury. The probe can be used to evaluate the burst of endogenous superoxide anion (O2 . − ) in mitochondria during cell I/R process. Further, to explore the relationship between O2 . − concentration and I/R injury degree. Ischemic preconditioning and postconditioning can limit the degree of liver I/R injury through the decrease of O2 . − burst.