Identification of potential early biomarkers of preeclampsia. (January 2018)

• Record Type: Journal Article
• Authors: Timofeeva, Angelika V.; Gusar, Vladyslava A.; Kan, Nataliya E.; Prozorovskaya, Kseniya N.; Karapetyan, Anna O.; Bayev, Oleg R.; Chagovets, Vitaliy V.; Kliver, Sergei F.; Iakovishina, Daria Yu.; Frankevich, Vladimir E.; Sukhikh, Gennadiy T.
• Is Part Of: Placenta. Volume 61(2018)
• Page Start: 61
• Page End: 71
• Publication Date: 2018-01
• Subjects: Preeclampsia -- NGS -- RT-PCR -- miRNA -- Placenta -- Blood plasma; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2017.11.011
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: Introduction: It is thought that poor placental perfusion caused by inadequate remodelling of the maternal spiral arteries leads to preeclampsia (PE). To identify novel signalling pathways that contribute to PE pathogenesis and to create prerequisites for the non-invasive diagnosis of PE before clinical manifestations of the disease, this study aimed to evaluate miRNA expression levels in the placenta and blood plasma of pregnant women. Methods: miRNA deep sequencing followed by real-time quantitative RT-PCR was applied to compare miRNA expression profiles in the placenta and blood plasma from women with early- and late-onset PE relative to the control group. Results: A more than two-fold decrease in miR-532-5p, -423-5p, -127-3p, -539-5p, -519a-3p, and -629-5p and let-7c-5p expression levels was observed in the placenta, while a more than two-fold increase in miR-423-5p, 519a-3p, and -629-5p and let-7c-5p was observed in the blood plasma of pregnant women with PE. The above-listed miRNAs are associated with PE for the first time in this study, except for miR-519a-3p, whose role in PE has already been postulated. Using a logistic regression, plasma samples were classified into the early-onset PE group (probability p = 0.01, 80% specificity, 87.5% sensitivity and 87.5% precision) and showed increased miR-423-5p expression levels that were confirmed by the 9.8-fold up-regulation (р = 0.0002498) of miR-423-5p expression observed in the blood plasma at 11–13 GW by RT-PCR in a group of pregnant women manifesting severe PE clinical signs at 28–33 GW. Conclusions: miR-423-5p may be considered a potential candidate for the early diagnosis of PE during the targeted management of high-risk pregnancies. Highlights: The expression of identified miRNAs depends on the gestational age. Early and late-onset PE differ in their miRNA expression levels. Placental miR-423-5p, -629-5p, and -519a-3p and let-7c-5p levels are increased in the blood plasma in early-onset PE. miR-423-5p was proposed as a potential candidate for the early diagnosis of PE.