Dispersal of single and mixed non-albicans Candida species biofilms by β-1, 3-glucanase in vitro. (December 2017)

• Record Type: Journal Article
• Authors: Tan, Yulong; Leonhard, Matthias; Ma, Su; Moser, Doris; Schneider-Stickler, Berit
• Is Part Of: Microbial pathogenesis. Volume 113(2017)
• Page Start: 342
• Page End: 347
• Publication Date: 2017-12
• Subjects: β-1, 3-glucan -- Biofilm -- Non-albicans Candida species; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.10.057
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: β-1, 3-glucan plays a role in non- albicans Candida species biofilm formation and survival of biofilm Candida to stresses. In this study, we evaluated the antibiofilm activity of β-1, 3-glucanase, which can degrade poly-β(1 → 3)-glucose of non- albicans Candida species biofilms, on single and mixed species biofilm of non- albicans Candida species, including Candida tropicalis, Candida parapsilosis and Candida krusei. Biofilm by all tested species in microplate were dispersed more than 60%. β-1, 3-glucanase also detached mixed species biofilm in microplate and on medical material surface. β-1, 3-glucanase had no effect on Candida planktonic growth as well as adhesion. However, further biofilm formation was inhibited with β-1, 3-glucanase added at 24 h after biofilm initiation. β-1, 3-glucanase markedly enhanced the antifungal susceptibility of amphotericin B. The examination using confocal laser scanning microscopy and scanning electron microscope confirmed the antibiofilm activity of β-1, 3-glucanase. Our findings demonstrate that β-1, 3-glucanase may be useful as an antibiofilm agent. Highlights: β-1, 3-glucanase disrupted single and mixed biofilm of non- albicans Candida species. β-1, 3-glucanase enhanced the antifungal susceptibility of amphotericin B. β-1, 3-glucanase may be useful as an antibiofilm agent.