Secretoneurin and PE-11 immunoreactivity in the human dental pulp. (February 2018)

• Record Type: Journal Article
• Authors: Steiner, René; Fischer-Colbrie, Reiner; Bletsa, Athanasia; Laimer, Johannes; Troger, Josef
• Is Part Of: Archives of oral biology. Volume 86(2018)
• Page Start: 13
• Page End: 17
• Publication Date: 2018-02
• Subjects: Secretoneurin -- PE-11 -- Sensory nerves -- Human dental pulp; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2017.11.005
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: Secretoneurin and PE-11 are chromogranin-derived peptides. Secretoneurin is a constituent of the sensory innervation of the dental pulp. PE-11 is a constituent of the sensory innervation of the dental pulp. The concentration of secretoneurin is not increased in the inflamed dental pulp. Abstract: Objective: To explore whether there are differences in the concentration of the secretogranin II-derived peptide secretoneurin and the chromogranin B-derived peptide PE-11 between the healthy and inflamed human dental pulps. Furthermore, colocalization studies with calcitonin gene-related peptide were performed to confirm the sensory origin of the peptidergic nerves in the dental pulp. Design: The concentrations of secretoneurin and PE-11 were determined by highly sensitive radioimmunoassays in extracts of dental pulps, the molecular form of secretoneurin immunoreactivities by RP-HPLC with subsequent radioimmunoassay and colocalization studies with calcitonin gene-related peptide were performed by double immunofluorescence. Results: Only secretoneurin but not PE-11 was detectable by radioimmunoassays whereas nerve fibers could be made visible for both secretoneurin and PE-11. Furthermore, there was a full colocalization of secretoneurin and PE-11 with calcitonin gene-related peptide in immunohistochemical experiments. There were no differences in the concentration of secretoneurin between the healthy and inflamed human dental pulp and moreover, the characterization of the secretoneurin immunoreactivities revealed that only authentic secretoneurin was detected with the secretoneurin antibody. Conclusions: There is unequivocal evidence that secretoneurin and PE-11 are constituents of the sensory innervation of the human dental pulp and although not exclusively but are yet present in unmyelinated C-fibers which transmit predominantly nociceptive impulses. Secretoneurin might be involved in local effector functions as well, particularly in neurogenic inflammation, given that this is the case despite of unaltered levels in inflamed tissue.