Antimicrobial activity of Melaleuca alternifolia nanoparticles in polymicrobial biofilm in situ. (December 2017)

• Record Type: Journal Article
• Authors: de Souza, Márcia Ebling; Clerici, Dariane Jornada; Verdi, Camila Marina; Fleck, Gabriela; Quatrin, Priscilla Maciel; Spat, Luana Ebling; Bonez, Pauline Cordenonsi; Santos, Camilla Fillippi dos; Antoniazzi, Raquel Pippi; Zanatta, Fabrício Batistin; Gundel, André; Martinez, Diego Stefani Teodoro; de Almeida Vaucher, Rodrigo; Santos, Roberto Christ Vianna
• Is Part Of: Microbial pathogenesis. Volume 113(2017)
• Page Start: 432
• Page End: 437
• Publication Date: 2017-12
• Subjects: Antibiofilm -- Chlorhexidine 0.12% -- Nanostructuring -- Tea tree -- Volatile oil -- AFM; EPS exopolysaccharides -- TTO Melaleuca alternifolia -- NPTTO M. alternifolia oil nanoparticles -- CHX chlorhexidine -- PDI polydispersity index -- SLN solid lipid nanoparticles -- AFM Atomic force microscopy; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.11.005
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Microbial biofilms represent a challenge in the treatment of infections, due to the low efficacy of the antimicrobials. This study evaluated the antimicrobial effect of nanoparticles of Melaleuca alternifolia (TTO) in dental biofilm. Thirty-eight volunteers used an oral device in situ in situ including four bovine enamel specimens for 07 days. From the fifth day four solutions were applied randomly for each specimen: Physiological Saline Solution (0.85% NaCl) (C+), Chlorhexidine 0.12% (CHX), M. alternifolia oil 0.3% (TTO), and a nanoparticle solution of 0.3% M. alternifolia oil (NPTTO). The nanoparticles of TTO were characterized for pH, IPD, medium size, zeta potential and Transmission Electron Microscopy. Antimicrobial activity was evaluated by viable microorganisms count and the structure of the biofilm by atomic force microscopy. The NPTTO presented pH 6.4, particle diameter of 197.9 ± 1 nm, polydispersion index of 0.242 ± 0.005, zeta potential of −7.12 mV and ±0:27 spherical shape. The C+ resulted in 100% of bacterial vitality, while CHX, TTO and NPTTO showed 34.2%, 51.4% and 25.8%, respectively. The AFM images showed biofilms with an average roughness of 350 nm for C+, 275 nm for CHX, 500 nm for TTO and 100 nm for NPTTO. The NPTTO demonstrated excellent antimicrobial activity in the biofilm formed in situ and will possibly be used in future for the treatment/prevention of oral biofilms.