Anal HPV 16 and 18 viral load: A comparison between HIV‐negative and ‐positive MSM and association with persistence. Issue 1 (17th October 2017)

• Record Type: Journal Article
• Authors: Marra, Elske; King, Audrey; van Logchem, Elske; van der Weele, Pascal; Mooij, Sofie H.; Heijman, Titia; Meijer, Chris J.L.M.; Verhagen, Dominique W.M.; van der Sande, Marianne A. B.; Schim van der Loeff, Maarten F.
• Is Part Of: Journal of medical virology. Volume 90:Issue 1(2018)
• Page Start: 76
• Page End: 83
• Publication Date: 2017-10-17
• Subjects: anal -- HIV -- HPV -- men who have sex with men -- persistence -- viral load; Virology -- Periodicals; 616
• DOI: 10.1002/jmv.24898
• Languages: English
• ISSNs: 0146-6615

Abstract

Abstract : Does anal HPV viral load explain the difference in anal HPV persistence between HIV‐negative and ‐positive men who have sex with men (MSM)? MSM ≥18 years were recruited in Amsterdam, the Netherlands, in 2010‐2011. Anal self‐swabs were collected every 6 months and genotyped (SPF10 ‐PCR‐DEIA‐LIPA25 ‐system). HPV16 and HPV18 load was determined with a type specific quantitative (q)PCR, and compared between HIV‐negative and ‐positive men using ranksum test. Persistence was defined as ≥3 positive samples for the same HPV‐type. Determinants of persistent HPV16/18 infection and its association with HPV16/18 load were assessed with logistic regression. Of 777 recruited MSM, 54 and 22 HIV negative men were HPV16 and HPV18 positive at baseline, and 64 and 39 HIV‐positive MSM. The geometric mean titer (GMT) of HPV16 was 19.6 (95%CI 10.1‐38.0) and of HPV18 8.6 (95%CI 2.7‐27.5) DNA copies/human cell. HPV16 and HPV18 load did not differ significantly between HIV‐negative and ‐positive MSM ( P = 0.7; P = 0.8, respectively). In multivariable analyses HPV16 load was an independent determinant of HPV16 persistence (OR 1.8, 95%CI 1.3‐2.4). No difference in anal HPV viral load was found between HIV‐positive and HIV‐negative MSM. HPV 16/18 viral load is an independent determinant of type‐specific persistence.