Bioenergetic adaptations of the human liver in the ALPPS procedure – how liver regeneration correlates with mitochondrial energy status. Issue 12 (December 2017)

• Record Type: Journal Article
• Authors: Alexandrino, Henrique; Rolo, Anabela; Teodoro, João S.; Donato, Henrique; Martins, Ricardo; Serôdio, Marco; Martins, Mónica; Tralhão, José G.; Caseiro Alves, Filipe; Palmeira, Carlos; Castro e Sousa, Francisco
• Is Part Of: HPB. Volume 19:Issue 12(2017)
• Page Start: 1091
• Page End: 1103
• Publication Date: 2017-12
• Subjects: Liver -- Diseases -- Periodicals; Biliary tract -- Diseases -- Periodicals; Pancreas -- Diseases -- Periodicals; 616.362005
• DOI: 10.1016/j.hpb.2017.08.005
• Languages: English
• ISSNs: 1365-182X

Abstract

Abstract : Background: The Associating Liver Partition and Portal Ligation for Staged Hepatectomy (ALPPS) depends on a significant inter-stages kinetic growth rate (KGR). Liver regeneration is highly energy-dependent. The metabolic adaptations in ALPPS are unknown. Aims: i) Assess bioenergetics in both stages of ALPPS (T1 and T2) and compare them with control patients undergoing minor (miHp) and major hepatectomy (MaHp), respectively; ii) Correlate findings in ALPPS with volumetric data; iii) Investigate expression of genes involved in liver regeneration and energy metabolism. Methods: Five patients undergoing ALPPS, five controls undergoing miHp and five undergoing MaHp. Assessment of remnant liver bioenergetics in T1, T2 and controls. Analysis of gene expression and protein content in ALPPS. Results: Mitochondrial function was worsened in T1 versus miHp; and in T2 versus MaHp (p < 0.05); but improved from T1 to T2 (p < 0.05). Liver bioenergetics in T1 strongly correlated with KGR (p < 0.01). An increased expression of genes associated with liver regeneration (STAT3, ALR) and energy metabolism (PGC-1α, COX, Nampt) was found in T2 (p < 0.05). Conclusion: Metabolic capacity in ALPPS is worse than in controls, improves between stages and correlates with volumetric growth. Bioenergetic adaptations in ALPPS could serve as surrogate markers of liver reserve and as target for energetic conditioning.