Identification and characterization of the lamprey Flotillin-1 gene with a role in cell adhesion. Issue 71 (December 2017)

• Record Type: Journal Article
• Authors: Xu, Rong; Song, Xiaoping; Su, Peng; Pang, Yue; Li, Qingwei
• Is Part Of: Fish & shellfish immunology. Issue 71(2017)
• Page Start: 286
• Page End: 294
• Publication Date: 2017-12
• Subjects: Flotillin-1 -- Lipid rafts -- Lamprey -- Cell adhesion; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.06.061
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Flotillin-1 is a kind of localize into specific cholesterol rich microdomains in cellular membranes and highly conserved lipid rafts marker protein widely distributed in animals and plants. It provides a platform for the reaction of many proteins in signal transduction, as scaffolding plays an important role in transmembrane signaling and cell adhesion. Here, Flotillin-1 protein from lamprey was identified and characterized (designated as L-Flotillin-1). After a partial cDNA sequence of L-Flotillin-1 was identified in a lamprey supraneural body cDNA library, the full-length cDNA was obtained using 3'- and 5'-rapid amplification of cDNA ends (RACE). L-Flotillin-1 encodes 424 amino acids and contains a prohibitin domain and a flotillin repetitive area. The L-Flotillin-1 protein was primarily distributed in kidney, supraneural body, gill, heart, liver and intestine via real-time PCR and immunohistochemistry assays. Immunofluorescence and western blot results showed that L-Flotillin-1 was considered to be used as a marker protein of lamprey lipid rafts and exosomes. Furthermore, overexpression of pEGFP-N1-L-Flotillin-1 induced the up-regulation of vascular cell adhesion molecule-1 (VCAM-1) and intercellular cell adhesion molecule-1 (ICAM-1) mRNA levels. These results indicated that the L-Flotillin-1 gene encodes Flotillin-1 protein that was used as a conserved marker protein and may play an important role in cell adhesion, providing clues for understanding the universal functions of Flotillin-1 proteins in other species and suggesting that these proteins could serve as pattern recognition molecules in immunotherapy. We revealed that Flotillin-1 protein of lamprey overexpression in human cells plays a prevalent role in cell migration and provide new thought of treatment to diseases. Highlights: Flotillin-1 gene was identified from lamprey supraneural body cDNA library. With continuous LPS stimulation, the level of Flotillin-1 protein expression was significant increase in intestinal tissue. The overexpression of pEGFP-N1-L-Flotillin-1 induced the up-regulation of VCAM-1 and ICAM-1 mRNA levels in Hela and HepG-2 cells.