A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda. Issue 71 (December 2017)

• Record Type: Journal Article
• Authors: Sun, Yuying; Zhang, Jiquan; Xiang, Jianhai
• Is Part Of: Fish & shellfish immunology. Issue 71(2017)
• Page Start: 43
• Page End: 49
• Publication Date: 2017-12
• Subjects: Exopalaemon carinicauda -- Chitinase -- CRISPR/Cas9 -- Hepatopancreas; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.09.065
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Chitinase, belonging to family 18 glycosyl hydrolase, is a multi-gene family and it has many functions. Generation of loss-of-function mutant targeting an interesting gene is a common way to clarify its function based on reverse genetics. In this study, we first reported the immune defense of a chitinase gene ( EcChi4 ) in Exopalaemon carinicauda using its EcChi4 -deletion mutant. EcChi4 was predominantly expressed in hepatopancreas and was upregulated after challenge with Vibrio parahaemolyticus or Aeromonas hydrophila . After knockout EcChi4 gene using CRISPR/Cas9 tool, the prawns in EcChi4 -deletion group had significant higher mortality than those in wild-type group when the prawns were challenged with V. parahaemolyticus or A. hydrophila . In conclusion, we first demonstrate the function of a chitinase gene in immune defense of E. carinicauda by performing directed, heritable gene mutagenesis. In the future, CRISPR/Cas9 should be widely applicable as a feasible means for gene editing in E. carinicauda for the study of important biological questions that cannot be easily addressed in other decapods. Highlights: EcChi4 was predominantly expressed in hepatopancreas of E. carinicauda. EcChi4 was upregulated after challenge with V. parahaemolyticus or A. hydrophila. EcChi4 -deleted prawn had higher mortality than wild-type after bacterial challenge. We first demonstrate the gene function of prawn by performing gene mutagenesis.