Measurement of 13C turnover into glutamate and glutamine pools in brain tumor patients. Issue 21 (27th October 2017)

• Record Type: Journal Article
• Authors: Pichumani, Kumar; Mashimo, Tomoyuki; Vemireddy, Vamsidhara; Ijare, Omkar B.; Mickey, Bruce E.; Malloy, Craig R.; Marin‐Valencia, Isaac; Baskin, David S.; Bachoo, Robert M.; Maher, Elizabeth A.
• Is Part Of: FEBS letters. Volume 591:Issue 21(2017)
• Page Start: 3548
• Page End: 3554
• Publication Date: 2017-10-27
• Subjects: [1, 2‐13C]acetate -- 13C isotopomer -- acetyl‐CoA -- glutamate and glutamine synthesis -- peripheral metabolism; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.12867
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Malignant brain tumors are known to utilize acetate as an alternate carbon source in the citric acid cycle for their bioenergetics. 13 C NMR‐based isotopomer analysis has been used to measure turnover of 13 C‐acetate carbons into glutamate and glutamine pools in tumors. Plasma from the patients infused with [1, 2‐ 13 C]acetate further revealed the presence of 13 C isotopomers of glutamine, glucose, and lactate in the circulation that were generated due to metabolism of [1, 2‐ 13 C]acetate by peripheral organs. In the tumor cells, [4‐ 13 C] and [3, 4‐ 13 C]glutamate and glutamine isotopomers were generated from blood‐borne 13 C‐labeled glucose and lactate which were formed due to [1, 2‐ 13 C[acetate metabolism of peripheral tissues. [4, 5‐ 13 C] and [3, 4, 5‐ 13 C]glutamate and glutamine isotopomers were produced from [1, 2‐ 13 C]acetyl‐CoA that was derived from direct oxidation of [1, 2‐ 13 C] acetate in the tumor. Major portion of C4 13 C fractional enrichment of glutamate (93.3 ± 0.02%) and glutamine (90.9 ± 0.03%) were derived from [1, 2‐ 13 C]acetate‐derived acetyl‐CoA. Abstract :