Trypsin of Litopenaeus vannamei is required for the generation of hemocyanin-derived peptides. (February 2018)

• Record Type: Journal Article
• Authors: Li, Changping; Wang, Fan; Aweya, Jude Juventus; Yao, Defu; Zheng, Zhou; Huang, He; Li, Shengkang; Zhang, Yueling
• Is Part Of: Developmental and comparative immunology. Volume 79(2018)
• Page Start: 95
• Page End: 104
• Publication Date: 2018-02
• Subjects: Litopenaeus vannamei -- Hemocyanin -- Peptides -- Trypsin -- Agglutination; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2017.10.015
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Hemocyanin is a copper containing respiratory glycoprotein in arthropods and mollusks, which also have multiple functions in vivo . Recent studies have shown that hemocyanin could generate several peptides, which play important roles in shrimp innate immunity. However, how these hemocyanin-derived peptides are generated is still largely unknown. In this study, we report for the first time that the generation of hemocyanin-derived peptides was closely correlated with trypsin expression in shrimp hepatopancreas following infection with different bacteria. RNA interference (RNAi) mediated knockdown of trypsin or treatment with the serine protease inhibitor, aprotinin, resulted in significant decrease in the levels of hemocyanin-derived peptides. Moreover, recombinant trypsin (rTrypsin) was able to hydrolyse hemocynin in vitro with the hydrolysate having a high bacterial agglutination activity while the denatured hemocynin untreated with rTrypsin lost its agglutination activity. Taken together, our current results showed that the generation of hemocyanin-derived peptides correlates with an increase trypsin expression. Highlights: First to show that Litopenaeus vannamei hemocyanin-derived peptides generation requires trypsin or a trypsin-like protease. Recombinant trypsin can digest hemocyanin in vitro, yielding a hydrolysate which possess bacterial agglutination activity.