Enzyme‐Triggered Fluorescence Turn‐on: A Probe for Specifically Imaging Ovarian‐Cancer‐Related γ‐Glutamyltranspeptidase. Issue 11 (1st August 2017)

• Record Type: Journal Article
• Authors: Tian, Jie; Yan, Qinglong; Zhu, Ying; Zhang, Jichao; Li, Jiao; Shi, Ben; Xu, Ge; Fan, Chunhai; Zhao, Chunchang
• Is Part Of: Chinese journal of chemistry. Volume 35:Issue 11(2017)
• Page Start: 1711
• Page End: 1716
• Publication Date: 2017-08-01
• Subjects: fluorescence turn‐on -- GGT -- the aromatic hydrocarbon transfer -- living cells -- specifically targeting; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/cjoc.201700248
• Languages: English
• ISSNs: 1001-604X

Abstract

Abstract : A fluorescent turn‐on probe for specifically targeting γ ‐glutamyltranspeptidase (GGT) was designed and synthesized by integrating boron‐dipyrromethene (BODIPY) as a chromophore and glutathione (GSH) as the GGT substrate. GGT‐catalyzed the cleavage of the γ ‐glutamyl bond and generated the aromatic hydrocarbon transfer between the sulfur and the nitrogen atom in BODIPY, leading to distinct optical changes. Such specific responsiveness provides an easily distinguishable fluorescence signal to visualize the GGT activity in living cells and differentiate GGT‐positive cancer cells from GGT‐negative cells. Abstract : GGT‐triggered fluorescence turn‐on was capable of differentiating GGT‐positive cancer cells from GGT‐negative cells.