Immobilization and stability studies of multi-thiol probes for electrical biosensors by label-free analysis. (2nd September 2017)

• Record Type: Journal Article
• Authors: Kim, Won Jae; Shim, Min Suk; Chung, Minsub
• Is Part Of: Molecular crystals and liquid crystals. Volume 654(2017)
• Page Start: 27
• Page End: 33
• Publication Date: 2017-09-02
• Subjects: Biosensor -- electrical detection -- impedance spectroscopy -- QCM-D -- thiol immobilization; Molecular crystals -- Periodicals; Liquid crystals -- Periodicals; Liquid crystals; Molecular crystals; Periodicals; 548
• DOI: 10.1080/15421406.2017.1355192
• Languages: English
• ISSNs: 1542-1406

Abstract

ABSTRACT: For electrical biosensors based on gold electrodes, poor long-term stability of the monothiol-to-gold bonds has been a major issue for a robust and reliable performance of the sensor. The use of multi-thiol modified bioprobes was proposed to achieve a stable attachment. In this study, the binding kinetics and stability of mono/dithiol modified oligonucleotides were investigated using quartz crystal microbalance with dissipation (QCM-D) and electrochemical impedance spectroscopy (EIS). These label-free surface tools compared the binding, stability and the effect of dithiothreitol in solution of monothiol, dithiol, or disulfide modification. The potential for development of label-free, sensitive DNA microarrays is demonstrated.